Incubation:Article Title: Increased urinary angiotensin converting enzyme 2 and neprilysin in patients with type 2 diabetes
Article Snippet: .. Resulting blots were then blocked and probed with goat anti-mouse ACE2 (1:1,000 dilution, R&D Systems), goat anti-mouse NEP/CD10 (1:1000 dilution, R&D Systems), rabbit anti-human ADAM17 (1:1,000 dilution, Abcam, Cambridge, MA), or goat anti-mouse albumin (1:1,000 dilution, Santa Cruz Biotechnology, Inc., Dallas, TX) primary antibodies followed by incubation with HRP-conjugated rabbit anti-goat secondary antibody (1:2,000, R&D Systems) or HRP conjugated goat anti-rabbit secondary antibody (1:20,000, Jackson, ME). .. Immobilon western chemiluminescent HRP substrate (Millipore) and autoradiography imaging method by Medical film processor (Konica Minolta Medical & Graphic, Inc., Wayne, NJ) were used to detect signals.
Article Title: Increased urinary angiotensin converting enzyme 2 and neprilysin in patients with type 2 diabetes.
Article Snippet: .. Resulting blots were then blocked and probed with goat anti-mouse ACE2 (1:1,000 dilution, R&D Systems), goat anti-mouse NEP/CD10 (1:1000 dilution, R&D Systems), rabbit anti-human ADAM17 (1:1,000 dilution, Abcam, Cambridge, MA), or goat antimouse albumin (1:1,000 dilution, Santa Cruz Biotechnology, Inc., Dallas, TX) primary antibodies followed by incubation with HRPconjugated rabbit anti-goat secondary antibody (1:2,000, R&D Systems) or HRP conjugated goat anti-rabbit secondary antibody (1: 20,000, Jackson, ME). .. Immobilon western chemiluminescent HRP substrate (Millipore) and autoradiography imaging method by Medical film processor (Konica Minolta Medical & Graphic, Inc., Wayne, NJ) were used to detect signals.
Article Title: Gut microbiota-derived metabolites confer protection against SARS-CoV-2 infection
Article Snippet: Antigen retrieval was conducted on tissues using Citrate Buffer Antigen Retriever (Sigma C9999) for 30 min at 85°C. .. After a wash step, sections were blocked with 5% horse serum in PBS for 60 min at room temperature, then incubated with goat anti-mouse ACE2 (1:200; R&D Systems AF3437) at 4°C overnight. .. After a wash step, sections were incubated with donkey anti-goat IgG H&L Alexa Fluor 488 antibody (1:200; Abcam ab150129) and counterstained with DAPI (1:5000) for 5 min. After staining, coverslips were mounted onto tissue sections using ProLongTM Gold Antifade Mountant (Invitrogen P36930).
Western Blot:Article Title: Expression and regulation of the neutral amino acid transporter B 0 AT1 in rat small intestine
Article Snippet: Analysis has been performed with the automated upright confocal laser scanning microscope SP8 (Leica) and image analysis has been performed with Imaris (Bitplane) software at the Center for Microscopy and Image Analysis (ZMB) of the University of Zürich. .. The following primary antibodies were used: rabbit anti-mouse B 0 AT1 antibody ([ ], 1:100 for IF, 1:2 000 for WB), goat anti-mouse ACE2 (R&D Systems, Minneapolis, MN, USA; dilution: 1:100 for IF, 1:1 000 for WB), rabbit anti-human CD13 (abcam, Cambridge, UK; dilution: 1:100 for IF, 1:2 000 for WB), and mouse anti-β-actin (Sigma Aldrich, Steinheim, Germany; dilution: 1:100 for IF and 1:5 000 for WB). .. Secondary antibodies were Anti-Rabbit IgG HRP Conjugate, Anti-Mouse IgG (H+L) AP Conjugate (Promega, Dübendorf, Switzerland) or donkey anti-goat IgG-HRP (Santa Cruz Biotechnology, Dallas, Texas, USA) for Western blot analysis (1:5 000 dilution) and Alexa Fluor ® 594 donkey anti-rabbit IgG (H+L) (life technologies, Carlsbad, California, USA) and Dnk pAb to Goat-IgG (DyLight ® 488) (abcam, Cambridge, UK) for immunofluorescence (1:2 000 dilution).
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